Twisted Vertex Operators and Unitary Lie Algebras A representation of the central extension of the unitary Lie algebra coordinated with a skew Laurent polynomial ring is constructed using vertex operators over an integral $\mathbb Z_2$-lattice. The irreducible decomposition of the representation is explicitly computed and described. As a by-product, some fundamental representations of affine Kac-Moody Lie algebra of type $A_n^{(2)}$ are recovered by the new method. Keywords:Lie algebra, vertex operator, representation theoryCategories:17B60, 17B69